@article {Govindarajan2017-mr,
	title = {Phenotypic heterogeneity in sugar utilization by E. coli is generated by stochastic dispersal of the general PTS protein EI from polar clusters},
	journal = {Front. Microbiol.},
	volume = {8},
	year = {2017},
	pages = {2695},
	abstract = {Although the list of proteins that localize to the bacterial cell poles is constantly growing, little is known about their temporal behavior. EI, a major protein of the phosphotransferase system (PTS) that regulates sugar uptake and metabolism in bacteria, was shown to form clusters at the Escherichia coli cell poles. We monitored the localization of EI clusters, as well as diffuse molecules, in space and time during the lifetime of E. coli cells. We show that EI distribution and cluster dynamics varies among cells in a population, and that the cluster speed inversely correlates with cluster size. In growing cells, EI is not assembled into clusters in almost 40\% of the cells, and the clusters in most remaining cells dynamically relocate within the pole region or between the poles. In non-growing cells, the fraction of cells that contain EI clusters is significantly higher, and dispersal of these clusters is often observed shortly after exiting quiescence. Later, during growth, EI clusters stochastically re-form by assembly of pre-existing dispersed molecules at random time points. Using a fluorescent glucose analog, we found that EI function inversely correlates with clustering and with cluster size. Thus, activity is exerted by dispersed EI molecules, whereas the polar clusters serve as a reservoir of molecules ready to act when needed. Taken together our findings highlight the spatiotemporal distribution of EI as a novel layer of regulation that contributes to the population phenotypic heterogeneity with regard to sugar metabolism, seemingly conferring a survival benefit.},
	keywords = {bacterial polarity, cell poles, dynamic localization, general PTS proteins, phenotypic heterogeneity, proteins localization, PTS system},
	author = {Govindarajan, Sutharsan and Albocher, Nitsan and Szoke, Tamar and Nussbaum-Shochat, Anat and Amster-Choder, Orna}
}
