@article {Kannaiah2019-vp,
	title = {Spatiotemporal organization of the E. coli transcriptome: Translation independence and engagement in regulation},
	journal = {Mol. Cell},
	volume = {76},
	number = {4},
	year = {2019},
	month = {nov},
	pages = {574{\textendash}589.e7},
	publisher = {Elsevier BV},
	abstract = {RNA localization in eukaryotes is a mechanism to regulate transcripts fate. Conversely, bacterial transcripts were not assumed to be specifically localized. We previously demonstrated that E. coli mRNAs may localize to where their products localize in a translation-independent manner, thus challenging the transcription-translation coupling extent. However, the scope of RNA localization in bacteria remained unknown. Here, we report the distribution of the E. coli transcriptome between the membrane, cytoplasm, and poles by combining cell fractionation with deep-sequencing (Rloc-seq). Our results reveal asymmetric RNA distribution on a transcriptome-wide scale, significantly correlating with proteome localization and prevalence of translation-independent RNA localization. The poles are enriched with stress-related mRNAs and small RNAs, the latter becoming further enriched upon stress in an Hfq-dependent manner. Genome organization may play a role in localizing membrane protein-encoding transcripts. Our results show an unexpected level of intricacy in bacterial transcriptome organization and highlight the poles as hubs for regulation.},
	keywords = {Bacteria, cell fractionation, E. coli, live-cell microscopy, RNA localization, RNA sequencing, small RNAs, spatial organization},
	author = {Kannaiah, Shanmugapriya and Livny, Jonathan and Amster-Choder, Orna}
}
